Immune responses of B. malayi thioredoxin (TRX) and venom allergen homologue (VAH) chimeric multiple antigen for lymphatic filariasis.
Identifieur interne : 003577 ( Main/Exploration ); précédent : 003576; suivant : 003578Immune responses of B. malayi thioredoxin (TRX) and venom allergen homologue (VAH) chimeric multiple antigen for lymphatic filariasis.
Auteurs : Gandhirajan Anugraha [Inde] ; Parasurama Jawaharlal Jeyaprita ; Jayaprakasam Madhumathi ; Tamilvanan Sheeba ; Perumal KalirajSource :
- Acta parasitologica [ 1896-1851 ] ; 2013.
Descripteurs français
- KwdFr :
- Agranulocytes (immunologie), Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (administration et posologie), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Brugia malayi (enzymologie), Brugia malayi (génétique), Brugia malayi (immunologie), Filariose lymphatique (), Filariose lymphatique (immunologie), Immunoglobuline G (sang), Interféron gamma (sécrétion), Interleukine-4 (sécrétion), Interleukine-5 (sécrétion), Mâle, Prolifération cellulaire, Protéines d'helminthes (administration et posologie), Protéines d'helminthes (génétique), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (immunologie), Souris, Souris de lignée BALB C, Thiorédoxines (génétique), Thiorédoxines (immunologie), Vaccins synthétiques (administration et posologie), Vaccins synthétiques (génétique), Vaccins synthétiques (immunologie).
- MESH :
- administration et posologie : Antigènes d'helminthe, Protéines d'helminthes, Vaccins synthétiques.
- enzymologie : Brugia malayi.
- génétique : Antigènes d'helminthe, Brugia malayi, Protéines d'helminthes, Protéines de fusion recombinantes, Thiorédoxines, Vaccins synthétiques.
- immunologie : Agranulocytes, Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Protéines de fusion recombinantes, Thiorédoxines, Vaccins synthétiques.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- sécrétion : Interféron gamma, Interleukine-4, Interleukine-5.
- Animaux, Filariose lymphatique, Mâle, Prolifération cellulaire, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antigens, Helminth (administration & dosage), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Brugia malayi (enzymology), Brugia malayi (genetics), Brugia malayi (immunology), Cell Proliferation, Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Helminth Proteins (administration & dosage), Helminth Proteins (genetics), Immunoglobulin G (blood), Interferon-gamma (secretion), Interleukin-4 (secretion), Interleukin-5 (secretion), Leukocytes, Mononuclear (immunology), Male, Mice, Mice, Inbred BALB C, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (immunology), Thioredoxins (genetics), Thioredoxins (immunology), Vaccines, Synthetic (administration & dosage), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology).
- MESH :
- chemical , administration & dosage : Antigens, Helminth, Helminth Proteins, Vaccines, Synthetic.
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , genetics : Antigens, Helminth, Helminth Proteins, Recombinant Fusion Proteins, Thioredoxins, Vaccines, Synthetic.
- chemical , immunology : Antigens, Helminth, Recombinant Fusion Proteins, Thioredoxins, Vaccines, Synthetic.
- enzymology : Brugia malayi.
- genetics : Brugia malayi.
- immunology : Brugia malayi, Elephantiasis, Filarial, Leukocytes, Mononuclear.
- prevention & control : Elephantiasis, Filarial.
- chemical , secretion : Interferon-gamma, Interleukin-4, Interleukin-5.
- Animals, Cell Proliferation, Male, Mice, Mice, Inbred BALB C.
Abstract
Although multiple vaccine strategy for lymphatic filariasis has provided tremendous hope, the choice of antigens used in combination has determined its success in the previous studies. Multiple antigens comprising key vaccine candidates from different life cycle stages would provide a promising strategy if the antigenic combination is chosen by careful screening. In order to analyze one such combination, we have used a chimeric construct carrying the well studied B. malayi antigens thioredoxin (BmTRX) and venom allergen homologue (BmVAH) as a fusion protein (TV) and evaluated its immune responses in mice model. The efficacy of fusion protein vaccine was explored in comparison with the single antigen vaccines and their cocktail. In mice, TV induced significantly high antibody titer of 1,28,000 compared to cocktail vaccine TRX+VAH (50,000) and single antigen vaccine TRX (16,000) or VAH (50,000). Furthermore, TV elicited higher level of cellular proliferative response together with elevated levels of IFN-γ, IL-4 and IL-5 indicating a Th1/Th2 balanced response. The isotype antibody profile showed significantly high level of IgG1 and IgG2b confirming the balanced response elicited by TV. Immunization with TV antigen induced high levels of both humoral and cellular immune responses compared to either cocktail or antigen given alone. The result suggests that TV is highly immunogenic in mice and hence the combination needs to be evaluated for its prophylactic potential.
DOI: 10.2478/s11686-013-0160-8
PubMed: 24338307
Affiliations:
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xml:lang="en"><term>Interferon-gamma</term><term>Interleukin-4</term><term>Interleukin-5</term></keywords><keywords scheme="MESH" qualifier="sécrétion" xml:lang="fr"><term>Interféron gamma</term><term>Interleukine-4</term><term>Interleukine-5</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Proliferation</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Filariose lymphatique</term><term>Mâle</term><term>Prolifération cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although multiple vaccine strategy for lymphatic filariasis has provided tremendous hope, the choice of antigens used in combination has determined its success in the previous studies. Multiple antigens comprising key vaccine candidates from different life cycle stages would provide a promising strategy if the antigenic combination is chosen by careful screening. In order to analyze one such combination, we have used a chimeric construct carrying the well studied B. malayi antigens thioredoxin (BmTRX) and venom allergen homologue (BmVAH) as a fusion protein (TV) and evaluated its immune responses in mice model. The efficacy of fusion protein vaccine was explored in comparison with the single antigen vaccines and their cocktail. In mice, TV induced significantly high antibody titer of 1,28,000 compared to cocktail vaccine TRX+VAH (50,000) and single antigen vaccine TRX (16,000) or VAH (50,000). Furthermore, TV elicited higher level of cellular proliferative response together with elevated levels of IFN-γ, IL-4 and IL-5 indicating a Th1/Th2 balanced response. The isotype antibody profile showed significantly high level of IgG1 and IgG2b confirming the balanced response elicited by TV. Immunization with TV antigen induced high levels of both humoral and cellular immune responses compared to either cocktail or antigen given alone. The result suggests that TV is highly immunogenic in mice and hence the combination needs to be evaluated for its prophylactic potential.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Jeyaprita, Parasurama Jawaharlal" sort="Jeyaprita, Parasurama Jawaharlal" uniqKey="Jeyaprita P" first="Parasurama Jawaharlal" last="Jeyaprita">Parasurama Jawaharlal Jeyaprita</name><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Madhumathi, Jayaprakasam" sort="Madhumathi, Jayaprakasam" uniqKey="Madhumathi J" first="Jayaprakasam" last="Madhumathi">Jayaprakasam Madhumathi</name><name sortKey="Sheeba, Tamilvanan" sort="Sheeba, Tamilvanan" uniqKey="Sheeba T" first="Tamilvanan" last="Sheeba">Tamilvanan Sheeba</name></noCountry><country name="Inde"><noRegion><name sortKey="Anugraha, Gandhirajan" sort="Anugraha, Gandhirajan" uniqKey="Anugraha G" first="Gandhirajan" last="Anugraha">Gandhirajan Anugraha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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